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April 12, 2020
Understanding HPLC Columns
There are various situations in life where you will need chemical applications. The discipline mostly deals with chemicals. There are various Chemistry courses provided at various institutions of higher learning.
One of the activities that is carried out in chemistry labs is the separation of items. The technique that is used to separate elements in a certain mixture is called high performance liquid chromatography. Column chromatography principles help in the separation of various elements. You will also find a mobile phase as well as a stationary phase in high performance liquid chromatography. Silica gel is preferred as a stationary phase in HPLC columns because it does not react with the mobile phases used in component separation. You need a computer for analysis of the separated, identified, and quantified elements of a mixture. It is possible to separate components in a mixture because they have different properties.
It is good for you to know how each part of a high performance liquid chromatography column works. You will find the solvent in the reservoir. Time is required for a certain to flow through the column, be separated, and be detected until it is displayed. High performance liquid chromatography column need UV light which is absorbed by different components at different wavelengths for easy detection.
Various HPLC columns of different nature are applied in chemistry analysis. The type of high performance liquid chromatography column you use will depend on the nature of the elements you are separating. Some types of HPLC columns are the size exclusion column, the reverse phase column, the normal phase column, and the ion exchange column. Normal phase HPLC columns ensure the separation of mixture components because some elements are absorbed by the less polar mobile phase while others are absorbed by the more polar stationary phase.
The reverse phase HPLC columns works on opposite normal phase HPLC columns. Common compounds used as stationary phase are non-polar hydrocarbons such C18 and C8 bonded hydrocarbons. The more polar organic components used as solvents in chromatography separation are aqueous organic solutions.
In ion exchange chromatography columns, components that are easily ionized are the ones that are analyzed. Stationary phase materials has positive or negative charge. The mobile phase in this types of HPLC column is supposed to be a polar material. You have to make sure that the components you are trying to separate can be separated by ionic exchange so that you use the ion exchange HPLC columns.
In the size exclusion HPLC column, there is a porous stationary phase which makes it possible to separate certain elements depending on their size. You are supposed to use a mixture of polymers to get a porous stationary material.
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